Biofilms essay

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Biofilms essay

Microbial biofilm Microbial biofilm Microbial biofilm are factions of surface-colonized cells encompassed in a matrix of self-secreted extracellular polymeric secretions.

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Antibiofilm activity of TzAgNPs was tested against Pseudomonas aeruginosa by carrying out an array of experiments like microscopic observation, crystal violet assay and protein count using the sub-MIC concentrations of TzAgNPs.

Since TzAgNPs showed efficient antibiofilm activity, thus, in the current study, we investigated the underlying cause of biofilm attenuation of Pseudomonas aeruginosa using TzAgNPs.

The result showed that the ROS level and microbial biofilm formation are inversely proportional. Thus, the attenuation in microbial biofilm could be attributed to the accumulation of ROS level. Furthermore, it was also duly noted that microorganisms upon treatment with TzAgNPs exhibited considerable diminution in virulence factors Protease and pyocyanin contrasted to control where the organisms were untreated with TzAgNPs.

Thus, the results indicated that TzAgNPs exhibits considerable reduction in the generation of biofilms and spreading of virulence factors.

Taken together, all the results indicated that TzAgNPs could be deemed to be a promising agent for the retardation of microbial biofilm that might assist to fight against infections linked to biofilm.

Pseudomonas aeruginosa, TzAgNPs, antimicrobial, antibiofilm, virulence factor. Biofilms essay Microorganisms live in nature in two forms; one is planktonic where the microorganism lives distantly free from each other while the other is known as biofilm where the microorganism lives in clustered colony form Garrett et al.

Biofilm can develop on both biotic and abiotic substances and are found in almost every environment Cortes et.

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Biofilm are Biofilms essay accumulation of microbial cells that are irreversibly adhered on a surface and confined within a layer of self-secreted extracellular polymeric substances EPS containing proteins, polysaccharides and DNA etc.

The EPS forms an interconnecting three-dimensional polymer network of cohesive nature which helps the microorganism to adhere to the surface as well as imbomilize biofilms cells transiently.

Microorganism adapts to the harsh condition by forming biofilm. It was reported that microbial biofilm is often involved in microbial pathogenesis Vasudevan Biofilm promotes gene transfer in the horizontal direction among bacterial population that steers an expansion in the population of virulent strains Lewis According to an estimation by the centre for disease control CDC and National Institutes of Health Joo and Otto,bacterial biofilm are involved in a multitude of serious, chronic infections, 65 and 80 percent of all infections, respectively.

Moreover, cells of the biofilm can not only escape the immunity of the host but can also stay unaffected by wide range of antibiotics Crossley et al. The infectious diseases caused by microbial biofilm include Endocarditis, Periodontitis, Osteomyelitis, Rhinosinusitis, Cystic fibrosis, etc Gupta et al.

Biofilm are also seen in medical implants, Joint prostheses, heart valves, Urinary catheters etc. Microbial biofilm often exhibits increased resistance to anti microbial agents.

A plausible explanation concerning the biofilm-refereed resistance to drugs could be accredited to the varied gene expression of the cells in the biofilm in comparison to the planktonic form of the same organism Whiteley et al.

Thus, biofilm cells can exhibit antibiotic resistance fold higher than the antibiotic resistance properties exhibited by planktonic cells Ceri et al. Among the various microbial pathogens capable of forming a biofilm, Pseudomonas aeruginosa can cause a biofilm on human host and is hence considered the most deadly.

Pseudomonas aeruginosa is responsible for several diseases including urinary tract and kidney infections Shirtliff and Mader ; Wagner and Iglewski The defence mechanism of microbial biofilm was found to be effective against a variety of antibiotics and disinfectants as illustrated in multiple publications.

In the light of the emergence of resistance to drugs, dissertations on new synthetic compounds having both antimicrobial and antibiofilm activity have attracted interest as an alternative potential drug against microbial pathogenesis Kumar et al.

Recent studies have showed that nano biotechnology have led to the development of newly creative therapeutic strategies and drug delivery alternatives particularly customized to deliver drugs to a selected site in order to enhance the efficacy of drug against microbial pathogenesis Conde et al.

Therefore, in the current study, we had synthesized 3,6-di pyridinyl -1,2,4,5-tetrazine pytz capped silver nanoparticles TzAgNPs in order to inhibit the formation of biofilm by Pseudomonas aeruginosa.

Materials and methods Synthesis of pytz and TzAgNPs Synthesis of 3,6-Di pyridinyl -1,4-dihydro-1,2,4,5-tetrazine H2pytz and 3,6-di pyridinyl -1,2,4,5-s-tetrazine pytz had been performed by following the protocol as described previously Audebert et al. In brief, the synthesis of 3,6-di pyridinyl -1,2,4,5-s-tetrazine pytz capped-silver nanoparticles TzAgNPs was achieved by the reduction of silver nitrate AgNO3 with 3,6-di pyridinyl -1,4-dihydro-1,2,4,5-tetrazine H2pytz.

A ml round bottom flask containing solution of 3,6-di pyridinyl -1,4-dihydro-1,2,4,5-tetrazine 0. After five minutes, a grey precipitate was slowly formed indicating the formation of silver nanoparticles TzAgNPs.

Essay: Biofilm - Essay UK Free Essay Database

The resulting particles were isolated by centrifugation 10 min, rpm and washed subsequently with water, ethanol and dichloromethane.

Microbial strain, growth media and culture conditions For the current study, Pseudomonas aeruginosa MTCC was used as a test organism. This organism was a kind gift to us from Dr.Biofilms Essay - A biofilm is a layer consisting of various combinations of many different organisms, autotrophic and heterotrophic.

They are dense, organized communities of . In this essay we will discuss the aetiology and symptoms of cystic fibrosis and the current available treatments, with particular emphasis on gene therapy and furanones, which prevent the build up of bacterial biofilms and thus reduce lung infection.

Nov 25,  · In the lower layers of a biofilm, microbes are bound together in a polysaccharide matrix with other organic and inorganic materials. Above it, is a loose amorphous layer extending into the surrounding medium.

Essay: Biofilm - Essay UK Free Essay Database

The fluid layer bordering the biofilm has stationary and dynamic sub layers. Free Essay: A biofilm is a layer consisting of various combinations of many different organisms, autotrophic and heterotrophic.

They are dense, organized. Biofilms are structured communities of bacteria that play a role in antibiotic resistant infections on catheters, which are tubes inserted through a narrow opening into a body cavity for removing fluids, and other medical prosthetic devices.

Biofilms Essay - A biofilm is a layer consisting of various combinations of many different organisms, autotrophic and heterotrophic.

Biofilms essay

They are dense, organized communities of cells, encased in a self-produced slime. The bacteria grow together in water like atmospheres, attaching to a solid surface, forming a small ecosystem.

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